Myoglobin Mouse Monoclonal Antibody

SKU: orb1816923

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Description

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Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:400-800, IF=1:100-500
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Mouse, Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	IgG
Clone No.	6H3
Immunogen	Recombinant human Myoglobin (1-154/154aa)
Target	MB
Molecular Weight	17 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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MYO; MB; MGC13548; PVALB; MYG_HUMAN; Recombinant human Myoglobin, Trx & His

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Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Myoglobin) Monoclonal Antibody, Unconjugated (orb1816923) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: Lane 1: Mouse Heart tissue lysates, Lane 2: Rat Muscle tissue lysates, Lane 3: Rat Heart tissue lysates, Primary: Anti-Myoglobin (orb1816923) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 17 kDa, Observed band size: 15 kDa.

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Gene Symbol

UniProt

P02144

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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