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NaK ATPase alpha 1 Rabbit Polyclonal Antibody
SKU: orb1294398
Description
Research Area
Cell Biology
Images & Validation
−Item 1 of 3
| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution Range | Western Blot 1:500-1:1000, Immunofluorescence 1:200-1:400, Immunohistochemistry (Paraffin) 1:100-1:200 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit-IgG |
| Immunogen | Synthetic peptide / encompassing a sequence within the N-terminus region. |
| Target | NaK ATPase alpha 1 / ATP1A1 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
anti-Na/K ATPase antibody at 1/700 dilution, Lysates/proteins at 40 µg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294398 overnight at 4°C.
Cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294398 anti-Na/K ATPase antibody (red) at 1:300 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded human cancer tissue labeling Na/K ATPase with orb1294398 at 1/100.
Quick Database Links
Gene Symbol
NaK ATPase alpha 1 / ATP1A1
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
NaK ATPase alpha 1 Rabbit Polyclonal Antibody (orb1294398)
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