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NCC Antibody
SKU: orb67566
Description
Research Area
Cancer Biology, Neuroscience, Pharmacology & Drug Discovery
Images & Validation
−Item 1 of 4
| Tested Applications | ICC, IEM, IF, IHC, WB |
|---|---|
| Dilution range | WB (1:1000), IHC-P (1:200) |
| Reactivity | Canine, Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Immunogen | Produced against a synthetic peptide mapping to a segment of rat NCC (amino acids 74-95), N-terminal |
| Target | NCC |
| Molecular Weight | 160kDa |
| Purification | Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−NCC, SLC12A3, SCYL1, CKb10, MCP-4, MGC17134, NCC-1, NCC1, SCYA13, CK-beta-10, Monocyte chemoattractant protein 4, Monocyte chemotactic protein 4, New CC chemokine 1, Small inducible cytokine A13, Small inducible cytokine subfamily A (Cys-Cys) member 13, Chemokine (C-C)
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Immunohistochemistry analysis using Rabbit Anti-NCC Polyclonal Antibody. Tissue: kidney tissue. Species: Rat. Primary Antibody: Rabbit Anti-NCC Polyclonal Antibody at 1:200. Secondary Antibody: FITC Goat Anti-Rabbit (green).
Western blot analysis of Rat tissue lysates showing detection of NCC protein using Rabbit Anti-NCC Polyclonal Antibody. Primary Antibody: Rabbit Anti-NCC Polyclonal Antibody at 1:1000.
Immunohistochemistry analysis using Rabbit Anti-NCC Polyclonal Antibody. Tissue: kidney tissue. Species: Mouse. Fixation: paraformaldehyde-fixed paraffin-embedded. Primary Antibody: Rabbit Anti-NCC Polyclonal Antibody at 1:500 for Overnight at 4°C. Secondary Antibody: Biotinylated Goat Anti-Rabbit IgG at 1:500 for 30 min at RT.
Immunohistochemistry analysis using Rabbit Anti-NCC Polyclonal Antibody. Tissue: kidney tissue. Species: Mouse. Fixation: paraformaldehyde-fixed paraffin-embedded. Primary Antibody: Rabbit Anti-NCC Polyclonal Antibody at 1:1000 for Overnight at 4°C. Secondary Antibody: Biotinylated Goat Anti-Rabbit IgG at 1:501 for 30 min at RT.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ICC
Immunocytochemistry
NCC Antibody (orb67566)
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