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NEU2 Antibody (N-term)
SKU: orb1937676
Description
Research Area
Signal Transduction
Images & Validation
−Item 1 of 7
| Tested Applications | FC, IF, IHC-P, WB |
|---|---|
| Dilution range | IHC-P-Leica - 1:250, WB - 1:2000 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 42254 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Disclaimer | For research use only |
Quality Guarantee
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Anti-NEU2 Antibody (N-term) at 1:2000 dilution + Human heart tissue lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 42 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Confocal immunofluorescent analysis of NEU2 Antibody (N-term) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Anti-NEU2 Antibody (N-term) at 1:1000 dilution + human fetal liver lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 42 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemical analysis on paraffin-embedded human kidney tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:250) for 15min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary Antibody.
Immunohistochemical analysis on paraffin-embedded human skeletal muscle tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:250) for 15min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary Antibody.
NEU2 Antibody (N-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
NEU2 Antibody (N-term) western blot analysis in A549 cell line lysates (35 ug/lane). This demonstrates the NEU2 antibody detected the NEU2 protein (arrow).
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UniProt
UniProt Details
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
IF
Immunofluorescence
NEU2 Antibody (N-term) (orb1937676)
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