NIFK rabbit pAb Antibody

SKU: orb770629

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Description

Rabbit polyclonal antibody to NIFK

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, IF, IHC, WB
Dilution range	WB 1:500-2000 Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Reactivity	Human, Mouse

Key Properties

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Clonality	Polyclonal
Isotype	IgG
Immunogen	The antiserum was produced against synthesized peptide derived from human NIFK. AA range:200-249
Molecular Weight	34
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Anti-MKI67IP antibody, anti-NIFK antibody, anti-NOPP34 antibody, anti-MKI67 FHA domain-interacting nucleolar phosphoprotein antibody, anti-Nucleolar phosphoprotein Nopp34 antibody, anti-Nucleolar protein interacting with the FHA domain of pKI-67 antibody, anti-hNIFK antibody

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Immunofluorescence analysis of HUVEC cells, using NIFK Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using NIFK Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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