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Nocodazole

SKU: orb1305003
FeaturedFeatured Product

Description

Nocodazole is a reversible microtubule polymerization inhibitor that also targets Bcr-Abl. This small molecule exhibits antitumor properties by arresting the cell cycle and triggering apoptosis, making it a valuable tool for in vitro cytoskeleton studies and cancer research.

Research Area

Cell Biology, Molecular Biology

Images & Validation

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Nocodazole

Key Properties

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CAS Number31430-18-9
MW301.32
Purity>98%
FormulaC14H11N3O3S
SMILESCOC(=O)Nc1nc2cc(ccc2[nH]1)C(=O)c1cccs1
TargetCytoskeleton
SolubilitySoluble in DMSO (up to 10 mg/ml).

Bioactivity

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Target IC50
MET:1.7 μM (Kd)|MEK1:1.7 μM (Kd)|Abl (T315I):0.64 μM|B-Raf (V600E):1.1 μM (Kd)|Abl (E255K):0.53 μM|c-Kit:1.6 μM (Kd)|Abl:0.21 μM|MEK2:1.6 μM (Kd)|PI3Kγ:1.5 μM (Kd)|B-Raf:1.8 μM (Kd)
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In Vivo
METHODS: To detect anti-tumor activity in vivo, Nocodazole (12 mg/kg three times a week) and dexamethasone (2 mg/kg twice a week) were injected intraperitoneally for fifteen days into immunodeficient mice bearing myeloma H929. RESULTS: Nocodazole in combination with dexamethasone significantly inhibited myeloma tumor growth and prolonged survival. METHODS: To study the effects on intestinal mucositis, Nocodazole (5 mg/kg) and ketoconazole (50 mg/kg) were administered intraperitoneally three times a week for six weeks to nude mice harboring the human colorectal cancer tumor COLO 205. RESULTS: The antitumor effect of Nocodazole was significantly enhanced after six weeks of ketoconazole treatment.
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In Vitro
METHODS: Erythrocytes were treated with Nocodazole (15-60 µg/mL) for 48 h. Phosphatidylserine was detected using Annexin-V-FITC. RESULTS: Nocodazole treatment increased the percentage of phosphatidylserine exposed to erythrocytes, reaching statistical significance at 30 µg/mL. METHODS: hESCs cells were treated with Nocodazole (100 ng/mL) for 16 h and cell cycle profiles were analyzed using Flow Cytometry. RESULTS: Nocodazole treatment synchronized the cell cycle (>90% of cells in G2/M), while cells remained synchronized after release and moved evenly through the cell cycle for 24 h. At 2 h after removal of Nocodazole, the cells entered the G1 phase, with 70% of the cells in the G1 phase after 4 h, and 80% in the S phase after 12 h. The cells were treated with Nocodazole (100 ng/mL) for 16 h, and the cell cycle was analyzed by Flow Cytometry.
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Cell Research
Nocodazole is dissolved in a final concentration of 0.05% DMSO. Proteins are loaded at 50 μg/lane and separated by 12% (w:v) sodium dodecyl sulfate-polyacrylamide gel electrophoresis, blotted, and probed with antibodies for cyclin E, p53, p21/CIP1, p27/KIP1, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), cyclin A, cyclin D1, cyclin D3, cyclin B, CDK2, CDK4, and cytochrome C. Immunoreactive bands are visualized by incubating with the colorigenic substrates nitroblue tetrazolium and 5-bromo-4-chloro-3-indolyl-phosphate. The expression of GAPDH is used as the control for equal protein loading.
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Storage & Handling

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StorageRT
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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R-17934

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Compound Identifiers

PubChem CID
4122 ↗

Key Properties

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No computed properties available.

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Nocodazole (orb1305003)

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10 mg
¥ 2,210.00
50 mg
¥ 4,030.00
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