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NTF3 Antibody (C-term)
SKU: orb1928914
Description
Images & Validation
−Item 1 of 5
| Tested Applications | FC, WB |
|---|---|
| Dilution range | FC - 1:25, WB - 1:2000 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Bovine, Gallus, Porcine, Xenopus |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 29355 Da |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Disclaimer | For research use only |
Quality Guarantee
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Anti-NTF3 Antibody (C-term) at 1:2000 dilution + NTF3 protein at 20 ng per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 13. 5 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-NTF3 Antibody (C-term) at 1:2000 dilution. Lane 1: Human brain lysate. Lane 2: Rat brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 29 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-NTF3 Antibody (C-term) at 1:2000 dilution. Lane 1: Human liver lysate. Lane 2: Human ovary lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 29 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-NTF3 Antibody (C-term) at 1:2000 dilution. Lane 1: Human ovary lysate. Lane 2: Human spleen lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 29 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Overlay histogram showing Hela cells stained (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed (1583138) at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
NTF3 Antibody (C-term) (orb1928914)
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