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Occludin Antibody
SKU: orb1294255
Description
Research Area
Cell Biology
Images & Validation
−Item 1 of 4
| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution range | Western Blot 1:500-1:1500 Immunofluorescence 1:200–1:300 Immunohistochemistry 1:100 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit-IgG |
| Immunogen | Synthetic peptide / Extracellular domain corresponding to Human Occludin / OCLN |
| Target | Occludin |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Disclaimer | For research use only |
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anti-Occludin antibody - at 1/1000 dilution, Lysates/proteins at 50 µg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294255 overnight at 4°C.
anti-Occludin antibody - at 1/1000 dilution, Lysates/proteins at 50 µg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294255 overnight at 4°C.
Immunohistochemical analysis of paraffin embedded mouse tissue labeling Occlidin with orb1294255 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
Immunohistochemical analysis of paraffin embedded mouse tissue labeling Occlidin with orb1294255 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
Quick Database Links
Gene Symbol
Occludin
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
Occludin Antibody (orb1294255)
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