OLIG2 Rabbit Polyclonal Antibody

SKU: orb574365

Description

Rabbit polyclonal antibody to OLIG2

Images & Validation

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Item 1 of 5

Tested Applications	IHC, WB
Reactivity	Human
Predicted Reactivity	Canine, Equine, Guinea pig, Mouse, Rat

Related Conjugates & Formulations

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Biotin

FITC

HRP

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	The immunogen is a synthetic peptide directed towards the C-terminal region of Human OLIG2
Target	OLIG2
Molecular Weight	32 kDa
Purification	Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration	0.5 mg/ml
Disclaimer	For research use only

Alternative Names

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BHLHB1, OLIGO2, RACK17, PRKCBP2, bHLHe19

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Sample Tissue: Human 293T, Antibody dilution: 1.0 ug/ml.

Sample Type: Fetal Lung, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 1 ug/ml, Peptide Concentration: 5 ug/ml, Lysate Quantity: 25 ug/lane, Gel Concentration: 0.12%.

OLIG2 Antibody - C-terminal region (orb574365) validated by WB using Fetal Lung cell lysate at 1.0 ug/ml.

Sample Type: Human Optic Nerve and Spinal CordCellular, dilution: 1:500.

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/ml in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

Quick Database Links

Gene Symbol

OLIG2

UniProt

Q13516

RefSeq (Protein):NP_005797

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_005797

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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