p150 CAF1 Rabbit mAb [KD Validated] Antibody

SKU: orb1950661

Description

Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer.

Images & Validation

−

Item 1 of 2

Tested Applications	FC, ICC, IHC, IP, WB
Dilution range	WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50 FC 1:500
Reactivity	Human

Key Properties

−

Antibody Type	Recombinant Antibody
Host	Rabbit
Clonality	Monoclonal
Isotype	Rabbit IgG
Immunogen	A synthesized peptide derived from human p150 CAF1
Purification	Affinity-chromatography
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Disclaimer	For research use only

Alternative Names

−

CAF; CAF1; CAF1P150; CHAF1A; DCAF1; hp15; P150;

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

p150 CAF1 Rabbit mAb [KD Validated] Antibody

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Western blot analysis of p150 CAF1 expression in K562 cell lysate.

Quick Database Links

UniProt

Q13111

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC

Immunohistochemistry

View Protocol

Flow Cytometry

View Protocol

ICC

Immunocytochemistry

View Protocol

Immunoprecipitation

View Protocol

Find More Protocols