p21 Waf1 Rabbit Polyclonal Antibody

SKU: orb1294337

Description

Rabbit polyclonal antibody to p21 Cip1

Research Area

Cell Biology

Images & Validation

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Item 1 of 6

Tested Applications	IF, IHC, WB
Dilution Range	Western Blot 1:500-1:1000, Immunofluorescence 1:200-1:400, Immunohistochemistry (Paraffin) 1:100–1:200
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit-IgG
Immunogen	Synthetic peptide / encompassing a sequence within the center region
Target	p21 Waf1 / Cip1
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	100mM Tris Glycine, 1% rAlbumin, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294337 anti-p21 Cip1 antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling p21 Cip1 with orb1294337 at 1/100.

p21 Cip1 antibody at 1/500 dilution. Lysates at 30 µg per lane. This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294337 overnight at 4°C.

Quick Database Links

Gene Symbol

p21 Waf1 / Cip1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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