p53 Rabbit pAb Antibody

SKU: orb765994

This product can be custom made

Featured

KO/KD
Validated

Description

Rabbit polyclonal antibody to p53

Research Area

Cancer Biology

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, IF, IHC, KO/KD Validated, WB
Dilution Range	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Reactivity	Human, Monkey, Mouse, Rat

Key Properties

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Clonality	Polyclonal
Isotype	IgG
Immunogen	The antiserum was produced against synthesized peptide derived from human p53. AA range:10-59
Molecular Weight	44
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Anti-TP53 antibody, anti-P53 antibody, anti-Cellular tumor antigen p53 antibody, anti-Antigen NY-CO-13 antibody, anti-Phosphoprotein p53 antibody, anti-Tumor suppressor p53 antibody

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Western blot analysis of lysates from 1) p53 knockout A431 cell, 2) A431 cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, Dylight 800 secondary antibody was diluted at 1:10000, 37°C 1 hour. (Red) Actin β Monoclonal Antibody (5B7) antibody was diluted at 1:5000 as loading control, 4°C over night, Dylight 680 secondary antibody was diluted at 1:10000, 37°C 1 hour.

Immunofluorescence analysis of human-liver tissue. 1, p53 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.

Immunofluorescence analysis of human-lung tissue. 1, p53 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.

Quick Database Links

UniProt

P04637

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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