p63 rabbit pAb Antibody

SKU: orb770715

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Description

Rabbit polyclonal antibody to p63

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, IF, IHC, WB
Dilution Range	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Reactivity	Human, Monkey, Mouse, Rat

Key Properties

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Clonality	Polyclonal
Isotype	IgG
Immunogen	The antiserum was produced against synthesized peptide derived from human p63. AA range:631-680
Molecular Weight	77
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Anti-TP63 antibody, anti-KET antibody, anti-P63 antibody, anti-P73H antibody, anti-P73L antibody, anti-TP73L antibody, anti-Tumor protein 63 antibody, anti-p63 antibody, anti-Chronic ulcerative stomatitis protein antibody, anti-CUSP antibody, anti-Keratinocyte transcription factor KET antibody, anti-Transformation-related protein 63 antibody, anti-TP63 antibody, anti-Tumor protein p73 antibody

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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Immunofluorescence analysis of Hela cell. 1, p63 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). β-tubulin Monoclonal Antibody (M7) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).

Immunofluorescence analysis of A549 cells, using p63 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from COS7 cells, using p63 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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