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PCNA antibody

SKU: orb1742620

Description

PCNA antibody recognizes the proliferating cell nuclear antigen (PCNA), a cofactor of DNA polymerase delta with a predicted molecular weight of 29 kDa. PCNA is important for DNA synthesis, repair, and epigenetic modifications, and also serves as an organelle marker of the nucleus. PCNA antibody can be used for distinguishing between early, middle, and late S-phase of the cell cycle as well as grading of different neoplasms.

Images & Validation

Tested ApplicationsFISH, ICC, IHC, IHC-Fr, IHC-P, IP, WB
Dilution rangeWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
ReactivityGolden Hamster, Human, Mouse, Rat
Application Notes
Positive control: NIH-3T3 , PC-12

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the center region of human PCNA. The exact sequence is proprietary.
Targetproliferating cell nuclear antigen
Molecular Weight29
PurificationPurified by antigen-affinity chromatography.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.025% ProClin 300. 1XPBS (pH7), 1% rAlbumin, 20% Glycerol
Concentration0.11 mg/ml (Please refer to the vial label for the specific concentration.)
DisclaimerFor research use only

Alternative Names

proliferating cell nuclear antigen , ATLD2

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

UniProt Details

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
IHC-Fr
Immunohistochemistry Frozen
View Protocol
ICC
Immunocytochemistry
View Protocol
IP
Immunoprecipitation
View Protocol

PCNA antibody (orb1742620)

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Available Sizes

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100 μl
¥ 8,060.00
DispatchUsually dispatched within 5-10 working days
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