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PCNA Antibody
SKU: orb345518
Description
Images & Validation
−Item 1 of 1
| Tested Applications | ELISA, IP, WB |
|---|---|
| Dilution range | ELISA: 1:10,000 - 1:50,000, IP: 1:100, WB: 1:5,000 - 1:32,000 |
| Reactivity | Algae |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 50-75 of Isochrysis galbana PCNA. |
| Purity | This affinity purified antibody is directed against algal PCNA. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest that this antibody would react with PCNA from the following alga sources: Prorocentrum minimum, Tetraselmis chui, Pleurochrysis carterae and Isochrysis galbana. Expect partial reactivity with PCNA from other alga sources as well as carrot, tobacco, peas, soybean and rice based on partial protein sequence homology. Species that have been tested but yielded no positive results are Pfiesteria piscicida, Emiliania huxleyi, Akashiwo sanguinea, Rhodomonas sp., Karlodinium micrum, and Skletonema costatum. Reactivity with PCNA from other sources has not been determined. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.42 mg/mL |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-PCNA antibody, Proliferating cell nuclear antigen
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Western blot using Biorbyt's Affinity Purified anti-Alga PCNA antibody shows detection of a predominant band at ~29 kDa corresponding to PCNA (lane 2). Peptide competition effectively eliminates specific band reactivity (lane 1). For peptide competition, the antibody was pre-incubated with 100 µg (excess) of the peptide for 2h at 37°C followed by overnight incubation at 4 °C with 100 µg (excess) of the peptide, followed by a brief centrifugation and then reaction with the membrane. Each lane contains a lysate from 105 cells from the marine dinoflagellate Prorocentrum minimum. Cells were separated by SDS-PAGE and transferred onto nitrocellulose using standard techniques. After blocking the membrane was probed with the primary antibody diluted to 1:200. Incubation was followed by washes and reaction with HRP Gt-a-Rabbit IgG [H&L] MX (orb347654). Detection was by ECL using a 30 sec exposure time. Other detection systems will yield similar results.
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IP
Immunoprecipitation
PCNA Antibody (orb345518)
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