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PCNA Antibody

SKU: orb1252133

Description

PCNA Antibody

Research Area

Cancer Biology, Cell Biology

Images & Validation

Tested ApplicationsIHC-P, WB
ReactivityHuman
Application Notes
Western blot: 1-2 µg/mLImmunohistochemistry (FFPE): 0.5-1 µg/mL for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Titering of the PCNA antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityMonoclonal
IsotypeIgG2a, kappa
Clone No.PM441-1
ImmunogenRecombinant proliferating cell nuclear antigen was used as the immunogen for the PCNA antibody.
TargetPCNA
PurificationProtein G affinity chromatography
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesPBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Concentration0.2 mg/mL
DisclaimerFor research use only

Alternative Names

Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA

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Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

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UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol

PCNA Antibody (orb1252133)

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100 μg
¥ 14,820.00
DispatchUsually dispatched within 5-10 working days
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