PCNA, monoclonal antibody

SKU: orb3145401

Description

Images & Validation

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Item 1 of 5

Tested Applications	IF, IHC, WB
Dilution Range	Western Blot 1:500 – 1:1000 Immunofluorescence 1:100 – 1:300 Immunohistochemistry (Paraffin) 1:50 – 1:150
Reactivity	Human, Mouse, Rat
Application Notes	Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	mouse IgG2a
Molecular Weight	35 kDa
Purification	Affinity column purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Concentration	0.5 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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proliferating cell nuclear antigen

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Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling PCNA with orb3145401 at 1/100.

Anti-PCNA antibody at 1/500 dilution, Lysates/proteins at 50 μg per lane.

Human paraffin-embedded intestine tissue. Citrate Buffer 1X pH6.0 at 95°C for 40 minutes. Labeling PCNA with orb3145401 at 1/100. The nuclei were counter staining with DAPI (blue).

PCNA antibody detects by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. PCNA antibody (orb3145401) diluted at 1:100.

PCNA antibody detects by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. PCNA antibody (orb3145401) diluted at 1:100.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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