Recombinant PD-L1 Antibody / B7-H1 / CD274

SKU: orb2635795

Description

PD-L1 is a checkpoint regulator in immune cells, it is expressed on immune or non-hematopoietic cells. Expression of the protein is seen during pregnancy where it has a role in suppressing the immune system. PD-L1 induces an inhibitory signal in activated T-cells and promotes T-cell apoptosis. It is overexpressed in a number of different cancers where it is believed to play a significant role in the cancer s ability to evade the immune system.

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Tested Applications	IHC-P, WB
Dilution range	Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 2-4ug/ml
Reactivity	Human
Application Notes	Optimal dilution of the recombinant PD-L1 antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	PDL1/8195R
Immunogen	A recombinant fragment (within amino acids 190-290) of human CD274 protein was used as the immunogen for the recombinant PD-L1 antibody.
Purification	Protein A/G affinity
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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Recombinant PD-L1 Antibody / B7-H1 / CD274

Western blot testing of human HDLM-2 cell lysate using recombinant PD-L1 antibody (clone rCD274/8195R). Predicted molecular weight ~34 kDa (unmodified), 45-70 kDa (glycosylated).

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UniProt

Q9NZQ7

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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