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PD-L1 Rabbit Polyclonal Antibody
SKU: orb1294268
Description
Research Area
Cancer Biology
Images & Validation
−Item 1 of 5
| Tested Applications | IF, IHC, IP, WB |
|---|---|
| Dilution Range | Western Blot & Immunoprecipitation: Reported in the literature, not verified in house., Immunofluorescence 1:100-1:300, Immunohistochemistry (Paraffin) 1:100-1:250 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit-IgG |
| Immunogen | Synthetic peptide / Intracellular domain corresponding to Human PD-L1 / CD274 |
| Target | PD-L1 / CD274 Intracellular domain (IHC prefer) |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 100mM Tris Glycine, 50% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. stained with orb1294268 anti-PD-L1 / CD274 antibody (red) at 1:200 and 4°C.
Cell: MDA-MB-231 1Ab: orb1294268 1-500 2Ab: anti Rabbit IgG (Light chain) 1-3000.
Immunohistochemical analysis of paraffin embedded Human breast cancer tissue labeling PD-L1 / CD274 with orb1294268 at 1/150.
Immunohistochemical analysis of paraffin embedded Human breast cancer tissue labeling PD-L1 / CD274 with orb1294268 at 1/150.
Immunohistochemical analysis of paraffin embedded Human breast cancer tissue labeling PD-L1 / CD274 with orb1294268 at 1/150.
Quick Database Links
Gene Symbol
PD-L1 / CD274 Intracellular domain (IHC prefer)
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
IP
Immunoprecipitation
PD-L1 Rabbit Polyclonal Antibody (orb1294268)
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