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PDGF-BB Recombinant Protein
Description
Research Area
Images & Validation
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Key Properties
−| Source | E. coli |
|---|---|
| Biological Activity | Biological activity was determined by the dose - dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED50 for this effect is 1.0 - 3.0 ng/mL. |
| Target | PDGFB |
| Protein Sequence | SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TVAAARPVT |
| Purity | Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg). |
Storage & Handling
−| Storage | The lyophilized PDGF-BB recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted PDGF-BB is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing PDGF-BB recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. |
|---|---|
| Form/Appearance | Lyophilized |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Human PDGF-BB Protein [orb1471774]
Unconjugated
Greater than 98% as determined by reducing SDS-PAGE.
12.42 KDa
Mammalian
10 μg, 50 μgRecombinant Human PDGF-BB Protein [orb3002491]
SDS-PAGE: Greater than 98% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified)
Predicted: 12.42 KDa. Observed: 14 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Mouse PDGF-BB Protein [orb3002372]
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 13.4 KDa. Observed: 15 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant human PDGF-BB protein (Active, CHO) [orb2978611]
≥ 95% as determined by SDS-PAGE.
12.2 kDa
500 μg, 10 μg, 50 μg

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Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_002599.1 |
|---|
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Protocol Information
PDGF-BB Recombinant Protein (orb1230932)
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