PHB Antibody (Center)

SKU: orb1931542

This product can be custom made

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cell Biology, Infectious Disease & Virology, Metabolism Research

Images & Validation

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Item 1 of 5

Tested Applications	FC, IF, IHC-P, WB
Dilution Range	IF - 1:10-50, WB - 1:1000, IHC-P - 1:10-50, FC - 1:10-50
Reactivity	Human

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Clone No.	BTD39735
Immunogen	This PHB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-117 amino acids from the Central region of human PHB. Antigen Region: 89-117 aa.
Target	PHB1 {ECO:0000303\|PubMed:28017329, ECO:0000312\|HGNC:HGNC:8912}
Molecular Weight	29804 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Prohibitin, PHB

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

PHB Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of PHB Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PHB antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of PHB Antibody (Center) in Hela cell line lysates (35 ug/lane). PHB (arrow) was detected using the purified Pab.

Western blot analysis of PHB (arrow) using rabbit polyclonal PHB Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PHB gene.