Phospho-ATF2 (Thr71) (G3) rabbit mAb Antibody

SKU: orb1946307

Description

Activating transcription factor 2 (ATF2), known as cyclic AMP (cAMP) response element (CRE) binding protein 2 (CREB2) and CRE-BP1 is a member of the activating protein-1 (AP1) transcription factor family. Through homo-dimerization or hetero-dimerization with other AP1 family members, such as the CREB, Fos, Maf, or Jun family transcription factor, it regulates the expression of many genes. ATF2 is an important mediator of mammalian cell responses to various stimuli, including stress. As a transcription factor, ATF2 acts as transducing extracellular signals to the nucleus to facilitate transcriptional responses to stimuli. ATF2 is stimulated by growth factors, ultraviolet (UV) radiation, and cytokines. Stress-activated protein kinases (SAPKs)(e.g. p38 MAP Kinase) induce transcriptional activation of ATF2. ATF2 is phosphorylated at amino acids threonine-69 and threonine-71 (T69, T71). Phosphorylation at these threonine residues is essential for maximal transcriptional activity downstream of insulin/epidermal growth factor stimulation and is dependent on cooperation between c-Jun N-terminal kinase (JNK) and signaling cascades downstream of Ras. Upon T69/T71 phosphorylation, ATF2 interacts with other AP1 proteins and translocate to the nucleus to regulate the expression of hundreds of genes.

Images & Validation

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Item 1 of 5

Tested Applications	FC, WB
Dilution range	1µg/mL - 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Reactivity	Human, Mouse

Key Properties

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Host	Rabbit
Clonality	Monoclonal
Isotype	Rabbit IgG1k
Clone No.	ATF2T71-G3
Immunogen	A synthetic phospho-peptide corresponding to residues surrounding Thr71 of human phospho ATF2
Purification	Protein A+G
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
Concentration	0.5 mg/mL
Disclaimer	For research use only

Alternative Names

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Cyclic AMP-dependent transcription factor ATF-2, Activating transcription factor 2, Cyclic AMP-responsive element-binding protein 2, CREB-2, HB16, CREB2, CREBP1

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Phospho-ATF2 (Thr71) (G3) rabbit mAb Antibody

Flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (blue) or 0.1 μg/mL of isotype control Cat. #orb1946241 (orange) or untreated (red) or treated with UV and PMA (green) using Phospho-ATF-2 (Thr71) antibody ATF2T71-G3 at 0.1μg/mL. Cat #orb1946307.

Western blot analysis of 293T cell extract untreated or treated with UV using 0.1 μg/mL Phospho-ATF-2 (Thr71) antibody ATF2T71-G3. Cat. #orb1946307.

Peptide blocking flow cytometric analysis of Jurkat cells secondary antibody only negative control (light blue) or untreated (red) or anisomycin-treated (green) or untreated and blocked with phospho-peptide (black) or anisomycin and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or anisomycin and blocked with non-phospho peptide (purple) Phospho-ATF-2 (Thr71) antibody ATF2T71-G3 at 0.1μg/mL. Cat. #orb1946307.

Flow cytometric analysis of Jurkat cells secondary antibody only negative control (blue) or untreated (red) or treated with anisomycin (green) using Phospho-ATF-2 (Thr71) antibody ATF2T71-G3 at 0.1μg/mL. Cat #orb1946307.

Flow cytometric analysis of Jurkat cells secondary antibody only negative control (blue) or untreated (red) or treated with anisomycin (green) using Phospho-ATF-2 (T71) antibody ATF2T71-G3 (Cat. #orb1946307) or Company C antibody at 0.07 μg/mL (manufacturer's recommended concentration).

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UniProt

P15336

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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