Phospho-HIST1H3B3(S10) Antibody

SKU: orb1931266

This product can be custom made

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Images & Validation

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Item 1 of 6

Tested Applications	DOT, IF, IHC-P, WB
Dilution Range	IF - 1:25, WB - 1:500, IHC-P-Leica - 1:100, DB - 1:500
Reactivity	Human

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Clone No.	BTD4895XTD4896
Immunogen	This HIST1H3B3 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S10 of human HIST1H3B3.
Target	H3C1 (HGNC:4766)
Molecular Weight	15404 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Histone H31, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, HIST1H3A, H3FA

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Dot blot analysis of anti-Phospho-HIST1 h3B3-S10 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hela cells labeling HIST1 h3A at 1/25 dilution, followed by Dylight 488-conjugated goat anti-Rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing Nucleus staining on Hela cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (red). The nuclear counter stain is DAPI (blue).

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Western blot analysis of lysates from Hela cell line, untreated or treated with nocodazole (1ug/ml, 18h), using Phospho-HIST1 h3B3 (S10) Antibody (upper) or Beta-actin (lower).

Western blot analysis of lysates from Hela cell line, untreated or treated with 20% FBS + 100nM Calyculin A, using Phospho-HIST1 h3B3 (S10) Antibody (upper) or Beta-actin (lower).