Phospho-Histone H3 (Ser10) Rabbit Polyclonal Antibody

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:2000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L, (HRP), Lysate: (-) HeLa, (+) HeLa + Nocodazole, (100 ng/ml, 18hr) + Calyculin A (100nM, 1hr), Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 17 kDa, Observed MW: 17 kDa.

IP of HeLa + Nocodazole (100 ng/ml, 18hr) + Calyculin A (100nM, 1hr) cells extracts, IP Ab incubation condition: orb1152070, 4°C, overnight, 1:25, 1:100 dilution, WB primary Ab incubation condition: orb1152070, room temperature 2h, 1:1000 dilution, Secondary Ab: Anti-Rabbit IgG for IP (HRP), Blocking buffer and Concentration: 5%, NFDM/TBST, Diluting buffer and Concentration: 5%, NFDM/TBST, Lane 1: 5% Input, Lane 2: IP with orb1152070 (1:25), Lane 3: IP with orb1152070 (1:100), Observed MW: 17 kDa, Exposure time: 30 s.

Tissue: Human tonsil, Section type: Formalin fixed & Paraffin-, embedded section, Retrieval method: High temperature and high, pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:2000, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: Anti-Rabbit and Mouse Polymer, HRP (Ready to use), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1152070.