Phospho-Histone H3 (Ser28) (D6) rabbit mAb Antibody

SKU: orb1946316

Description

Histones are highly conserved proteins that serve the core of nucleosomes, which serve to organize chromatin fiber for DNA packing. Histone H3 phosphorylation plays a major role in both transcriptional activation, which requires unpacking of the chromatin structure, and in chromosome packing during cell division. Histone H3 is phosphorylated at residues Ser10 and Ser28, and is acetylated at Lys14. Phosphorylation at Ser10 occurs during entry into mitosis prior to chromatin condensation, and phosphorylation at Ser28 follows a similar pattern. In response to EGF stimulation, it has been proposed that sequential Ser10 phosphorylation, then Lys14 acetylation occurs, causing a change in chromatin structure and gene activation.

Images & Validation

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Item 1 of 5

Tested Applications	FC, WB
Dilution range	1µg/mL - 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Reactivity	Human, Mouse

Key Properties

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Host	Rabbit
Clonality	Monoclonal
Isotype	Rabbit IgG1k
Clone No.	HisH3S28-D6
Immunogen	A synthetic phospho-peptide corresponding to residues surrounding Ser28 of human phospho Histone H3
Purification	Protein A+G
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
Concentration	0.5 mg/mL
Disclaimer	For research use only

Alternative Names

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Histone H3.1t, H3t, H3FT, HIST3H3

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Phospho-Histone H3 (Ser28) (D6) rabbit mAb Antibody

Flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (blue) or 0.1 μg/mL of isotype control Cat. #orb1946241 (orange) or untreated (red) or treated with UV and PMA (green) using Phospho-Histone H3 (Ser28) antibody HisH3S28-D6 at 0.1μg/mL. Cat #orb1946316.

Western blot analysis of Hela cell extract untreated or synchronized in metaphase by treatment with 0.4 ug/mL of nocodazole for 6 hr then metaphase cells were isolated by mitotic shake-off using Phospho-Histone H3 (Ser28) antibody at 1 ng/mL HisH3S28-D6. Cat. #orb1946316.

Flow cytometric analysis of Hela secondary antibody only negative control (blue) or untreated (red) or treated with nocodazole (green) using 0.05 μg/mL Phospho-Histone H3 (Ser28) antibody HisH3S28-D6. Cat. #orb1946316.

Flow cytometric analysis of HeLa cells secondary antibody only negative control (blue) or untreated (red) or treated with nocodazole (green) using Phospho-Histone H3 (Ser28) antibody HisH3S28-D6 (Cat. #orb1946316) at 0.05ug/mL or Company C antibody at 0.2ug/mL (manufacturer's recommended concentration).

Western blot analysis of HeLa cells untreated or treated with nocodazole (green) using Phospho-Histone H3 (Ser28) antibody HisH3S28-D6 (Cat. #orb1946316) at 0.005ug/mL or Company C antibody at 0.07ug/mL (manufacturer's recommended concentration) developed using the same exposure.

Quick Database Links

UniProt

Q16695

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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