Phospho-MSK1 (Thr581) (A5) rabbit mAb Antibody

SKU: orb1946223

Description

MSK1 (mitogen and stress activated protein kinase 1, phospho MSK1) is activated by Erk in response to growth factors and by and p38 in response to cellular stress (1). MSK1 is similar to RSK1 in that it has two kinase domains and a connecting regulatory linker region (2). S364/S381 phosphorylation activates RSK1 (3), which is analogous to residues S360 and S376 of MSK1, which may be important for phospho MSK1 activity.

Images & Validation

−

Item 1 of 6

Tested Applications	FC, WB
Dilution range	1µg/mL - 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Reactivity	Human, Mouse, Rat

Key Properties

−

Host	Rabbit
Clonality	Monoclonal
Isotype	Rabbit IgG1k
Clone No.	MSK1T581-A5
Immunogen	A synthetic phospho-peptide corresponding to residues surrounding Thr581 of human phospho MSK1
Purification	Protein A+G
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
Concentration	0.5 mg/mL
Disclaimer	For research use only

Alternative Names

−

Ribosomal protein S6 kinase alpha-5, S6K-alpha-5, 90 kDa ribosomal protein S6 kinase 5, Nuclear mitogen- and stress-activated protein kinase 1, RSK-like protein kinase, RSKL, RPS6KA5

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Phospho-MSK1 (Thr581) (A5) rabbit mAb Antibody

Western blot analysis of 293T cell extract untreated or treated with UV using 0.1 ug/mL Phospho-MSK1 (Thr581) antibody MSK1T581-A5. Cat. #orb1946223.

Flow cytometric analysis of U937 cells secondary antibody only negative control (blue) or untreated (red) or treated with CalA (green) using Phospho-MSK1 (T581) antibody MSK1T581-A5 1.0 μg/mL. Cat. #orb1946223.

Peptide blocking flow cytometric analysis of C6 cells secondary antibody only negative control (light blue) or pervanadate-treated and stained using 0.1μg/mL isotype control Cat. #orb1946241 (orange) or pervanadate-treated and stained using 0.1μg/mL Phospho-MSK1 (Thr581) antibody MSK1T581-A5 Cat. #orb1946223 (green) or pervanadate and blocked with phospho peptide (gold) or pervanadate and blocked with non-phospho peptide (purple).

Flow cytometric analysis of C2C12 cells secondary antibody only negative control (blue) or 0.1 μg/mL of isotype control Cat. #orb1946241 (orange) or treated with imatinib (red) or with pervanadate (green) using Phospho-MSK1 (Thr581) antibody MSK1T581-A5 at 0.1 μg/mL. Cat #orb1946223.

Peptide blocking flow cytometric analysis of K562 cells secondary antibody only negative control (light blue) or imatinib-treated (red) or pervanadate-treated (green) or imatinib and blocked with phospho-peptide (black) or pervanadate and blocked with phospho peptide (gold) or imatinib and blocked with non-phospho peptide (dark blue) or pervanadate and blocked with non-phospho peptide (purple) Phospho-MSK1 (Thr581) antibody MSK1T581-A5 at 0.1μg/mL. Cat. #orb1946223.

Western blot analysis of 293T cell extract untreated or treated with staurosporine using 0.1 μg/mL Phospho-MSK1 (Thr581) antibody MSK1T581-A5 Cat. #orb1946223 or Company C antibody at 0.02 μg/mL (manufacturer's recommended concentration) developed using the same exposure.

Quick Database Links

UniProt

O75582

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

Flow Cytometry

View Protocol

Find More Protocols