Phospho-SEK1/MKK4 (Ser257) (C5) rabbit mAb Antibody

SKU: orb1946068

Description

SEK1, in cooperation with MKK7, activates JNK by tyrosine phosphorylation in response to cellular stress signals, including osmotic stress, DNA damage, cytokines, and heat shock. SEK1 itself is activated by serine phosphorylation by MAP3Ks, including MEKK1, MLKs, ASK1, and TAK1. In response to TNFŒ± stimulation in melanoma cells, filamin interacts with SEK1 as a scaffold protein. SEK1 plays an important role in T cell signaling pathways. Both SEK1-dependent and independent pathways operate for SAPK activation in response to different cellular stresses and stimuli. SEK1 also plays an important role in embryonic development. SEK1 may activate c-Jun and c-Fos during mammalian embryonic liver development.

Images & Validation

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Tested Applications	FC, WB
Dilution Range	1µg/mL - 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Reactivity	Human, Monkey, Mouse

Key Properties

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Host	Rabbit
Clonality	Monoclonal
Isotype	Rabbit IgG1k
Clone No.	SEK1MKK4S257-C5
Immunogen	A synthetic phospho-peptide corresponding to residues surrounding Ser257 of human phospho SEK1/MKK4
Purification	Protein A+G
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
Concentration	0.5 mg/mL
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Dual specificity mitogen-activated protein kinase kinase 4, MAP kinase kinase 4, MAPKK4, JNK-activating kinase 1, MEK4, SAPKK1, JNKK1, PRKMK4, MAP2K4, SERK1, SKK1

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Phospho-SEK1/MKK4 (Ser257) (C5) rabbit mAb Antibody

Flow cytometric analysis of HEK293T cells treated with lambda phosphatase (red) or treated with UV + TPA (green) using Phospho-SEK1/MKK4 (Ser257) (C5) Rabbit mAb, at 0.1 ug/mL, SEK1MKK4S257-C5 #orb1946068, or concentration-matched Rabbit (G9) mAb IgG Isotype Control #orb1946241 for cells treated with phosphatase (black) or treated with UV + TPA (blue).

Flow cytometric analysis of 3T3 cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using Phospho-SEK1/MKK4 (Ser257) antibody SEK1MKK4S257-C5 at 0.1 μg/mL. Cat. #orb1946068.

Western blot analysis of THP1 cell extract, untreated or treated with sorbitol using phospho-SEK1/MKK4 (Ser257) antibody SEK1MKK4S257-C5 at 0.05 ug/mL. Cat. #orb1946068.

Western blot analysis of COS7 cell extract, untreated or treated with UV plus TPA using phospho-SEK1/MKK4 (Ser257) antibody SEK1MKK4S257-C5 at 0.05 ug/mL. Cat. #orb1946068.

Western blot analysis of THP1 cell extract untreated or treated with sorbitol using 0.05 μg/mL Phospho-SEK1/MKK4 (Ser257) antibody SEK1MKK4S257-C5 Cat. #orb1946068 or Company C antibody at 0.01 μg/mL (manufacturer's recommended concentration) developed using the same exposure.

Quick Database Links

UniProt

P45985

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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