Phospho-Zap70 (Tyr319)/Syk (Tyr352) (A3) rabbit mAb Antibody

SKU: orb1946288

Description

ZAP70 (Tyrosine-protein kinase ZAP-70, phospho Zap70) is a protein tyrosine kinase (PTK) that associates with the z subunit of the T cell antigen receptor (TCR) and undergoes tyrosine phosphorylation following TCR stimulation. Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck. ZAP70 contains two SH2-like domains with the PTK domain located at the C-terminus. It appears that both phospho Zap70 and Syk are recruited to the phosphorylated CD3 and z subunits after TCR stimulation. Phosphorylation of Tyr319 is required for the assembly of a phospho Zap70-containing signaling complex that leads to the activation of the PLC-gamma1-dependent and Ras-dependent signaling cascades in antigen-stimulated T cells. The orthologous Tyr352 residue in Syk is also involved in the association with PLC-gamma1.

Images & Validation

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Item 1 of 4

Tested Applications	FC
Dilution Range	1µg/mL - 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Reactivity	Human, Mouse

Key Properties

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Host	Rabbit
Clonality	Monoclonal
Isotype	Rabbit IgG1k
Clone No.	Zap70Y319-A3
Immunogen	A synthetic phospho-peptide corresponding to residues surrounding Tyr319/Tyr352 of human phospho Zap70/Syk.
Purification	Protein A+G
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
Concentration	0.5 mg/mL
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Tyrosine-protein kinase ZAP-70 , 70 kDa zeta-chain associated protein, Syk-related tyrosine kinase, SRK, Tyrosine-protein kinase SYK, Spleen tyrosine kinase, p72-Syk

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Phospho-Zap70 (Tyr319)/Syk (Tyr352) (A3) rabbit mAb Antibody

ZapY319-A3 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of L929 cells secondary antibody only (blue) or 0.1 μg/mL of isotype control Cat. #orb1946241 (orange) or of Phospho-Zap70 (Tyr319) /Syk (Tyr352) antibody ZapY319-A3 (green) Cat. #orb1946288.

Flow cytometric analysis of Jurkat cells secondary antibody only negative control (blue) untreated (red) treated with pervanadate (green) treated + blocked with non-phospho-peptide (violet) or treated + blocked with phospho-peptide (brown) using Phospho-Zap70 (Tyr319) /Syk (Tyr352) antibody ZapY319-A3 (0.05 ?μg/mL) Cat. #orb1946288.

Flow cytometric analysis of Jurkat cells secondary antibody only negative control (blue) or untreated (red) or treated with H2O2 (green) using 0.01 μg/mL Phospho-Zap70 (Y319) /Syk (Y352) antibody ZapY319-A3 (Cat. #orb1946288) or Company C antibody at 0.2 μg/mL (manufacturer's recommended concentration).

Flow cytometric analysis of Jurkat cells, untreated (red) or treated with pervanadate (green) using Phospho-Zap70 (Tyr319) /Syk (Tyr352) A3 antibody Zap70Y319-A3 at 0.01 μg/mL, Cat.#orb1946287 or concentration matched isotope (KLH-G9) for untreated cells (gray) or treated with pervandate (blue) Cat.#orb1946241 #orb1946019.

Quick Database Links

UniProt

P43405, P43403

UniProt Details

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No UniProt data available

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Protocol Information

Flow Cytometry

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