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Description
Research Area
Cell Biology
Images & Validation
−Item 1 of 5
| Tested Applications | IF, IHC-P, WB |
|---|---|
| Dilution Range | IF: 1:25, WB: 1:1000, IHC-P: 1:25, IHC-P: 1:25, IHC-P: 1:25 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Immunogen | Recombinant PINK1 protein was used to produced this monoclonal antibody. Antigen Region: Unknown. |
| Target | PINK1 |
| Molecular Weight | 62769 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Serine/threonine-protein kinase PINK1, mitochondrial, BRPK, PTEN-induced putative kinase protein 1, PINK1
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Immunohistochemical analysis of paraffin-embedded H. heart section using Pink1 (115-213). Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded Human colon cancer section using Pink1. Diluted at 1: 200 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded H. stomach section using Pink1 (115-213). Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
All lanes: Anti-Pink1 Antibody at 1:1000 dilution. Lane 1: A431 whole cell lysates. Lane 2: mouse brain lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 63 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Fluorescent image of PC12 cells stained with Pink1 (115-213) Antibody. Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_115785.1 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
PINK1 Antibody (orb1788045)
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