PMS2 (N) Antibody, Rabbit Polyclonal

SKU: orb131722

Description

Rabbit polyclonal to PMS2. Mismatch repair endonuclease PMS2 belongs to the DNA mismatch repair MutL/HexB family. PMS2 is a component of the post-replicative DNA mismatch repair system (MMR). PMS2 heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch.

Research Area

Epigenetics & Chromatin

Images & Validation

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Tested Applications	WB
Dilution Range	WB:1:1000-1:3000
Reactivity	Human, Mouse
Application Notes	WB: The apparent protein size on WB may be different from the calculated M.W. due to modifications. Experiment Notes: Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the N-terminal region of human PMS2.

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	N-terminal region of human PMS2
Molecular Weight	96
Purity	Affinity purification
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	0.01% NaN3
Concentration	100 μg/ml BSA
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti Mismatch repair endonuclease PMS2 antibody, anti DNA mismatch repair protein PMS2 antibody, anti PMS1 protein homolog 2 antibody, anti PMSL2 antibody

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UniProt

P54278

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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