Antibody Type	Primary Antibody
Host	Mouse
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human PPP3CA (15-80/521aa)
Target	PPP3CA
Molecular Weight	57 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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Calcineurin A alpha; CalcineurinA; Calcineurin-A; Calcineurin A; Calmodulin dependent calcineurin A subunit alpha isoform; CALN; CALNA 1; CALNA; CALNA1; CAM PRP catalytic subunit; CCN 1; CCN1; CNA 1; CNA; CNA1; PPP2B; Protein phosphatase 3(formerly 2B) catalytic subunit alpha isoform; Serine/threonine protein phosphatase 2B catalytic subunit alpha isoform; PP2BA_HUMAN; CAM-PRP catalytic subunit; Calmodulin-dependent calcineurin A subunit alpha isoform; protein phosphatase 2B, catalytic subunit, alpha isoform; serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform.

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MCF7 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (PPP3CA) polyclonal Antibody, Unconjugated (orb10217) 1:25, 90 minutes at 37°C, followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

The MCF-7 (H) cells were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Mouse Anti-PPP3CA antibody (orb10217): 2 µg/10^6 cells, Secondary Antibody (white blue): Goat anti-Mouse IgG-FITC: 1 µg/test. Isotype Control (orange): Mouse IgG. Blank control (black): PBS. Acquisition of 20000 events was performed.

Quick Database Links

Gene Symbol

PPP3CA

UniProt

Q08209

UniProt Details

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No UniProt data available

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Protocol Information

Flow Cytometry

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