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Rabbit anti-API5 Antibody
SKU: orb1525998
Description
Images & Validation
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| Tested Applications | IHC, IP, WB |
|---|---|
| Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Between 1 and 50 |
| Target | API5 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Whole IgG |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 200 µg/ml |
| Disclaimer | For research use only |
Alternative Names
−Fibroblast growth factor 2-interacting factor; Protein XAGL; migration-inducing protein MIG8; AAC11; fibroblast growth factor 2-interacting factor 2; cell migration-inducing gene 8 protein; apoptosis inhibitor 5; API-5; antiapoptosis clone 11 protein; AAC-11; FIF
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Detection of human and mouse API5 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-API5 antibody orb1525998 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human API5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat, cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-API5 antibody orb1525998 used for IP at 3 µg per reaction.
Detection of human API5 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-API5 (Cat. No. orb1525998) used at 1: 200 (1 µg/ml). Detection: DAB
Detection of mouse API5 by immunohistochemistry.Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-API5 (Cat. No. orb1525998) used at 1: 200 (1 µg/ml) Detection: DAB
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| RefSeq | XP_508375.2 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IP
Immunoprecipitation
Rabbit anti-API5 Antibody (orb1525998)
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