Rabbit CD247/CD3Z Recombinant Monoclonal Antibody

SKU: orb1519961

Description

CD247/CD3Z Antibody

Research Area

Cancer Biology, Immunology & Inflammation

Images & Validation

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Item 1 of 1

Tested Applications	FC, IHC, IP, WB
Dilution Range	WB - 1:1000; IP - 20 µl/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; IHC-IF - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Reactivity	Human
Predicted Reactivity	Mouse, Porcine, Sheep
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	BL-336-1B2
Immunogen	Between 114 and C-terminus
Target	CD247/CD3Z
Purification	Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	100 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CD antigen CD247; CD247 antigen, zeta subunit; CD3H; CD3Q; CD3; ZCD3Z antigen, zeta polypeptide (TiT3 complex); CD3-ZETA; CD3zeta chain; IMD25; T3; ZT-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor T3 zeta chain; T-cell surface glycoprotein CD3 zeta chain; TCR zeta chain; TCRZ

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Rabbit CD247/CD3Z Recombinant Monoclonal Antibody

Detection of human CD247/CD3Z by Simple Western. Samples: Whole cell lysate (2.0 mg/mL) from Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD247/CD3Z recombinant monoclonal antibody [BL-336-1B2] (orb1519961) used at 1:10, 1:50, 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram.

Quick Database Links

Gene Symbol

CD247/CD3Z

UniProt

P20963

RefSeq (Protein):NP_000725.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_000725.1

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Datasheet

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

Flow Cytometry

Immunoprecipitation