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Rabbit HEXIM1 Antibody
SKU: orb1525442
Description
Research Area
Cardiovascular Research
Images & Validation
−Item 1 of 3
| Tested Applications | IHC, IP, WB |
|---|---|
| Dilution Range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Bovine, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 200 and 250 |
| Target | HEXIM1 |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 200 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−cardiac lineage protein 1; CLP1; EDG1; estrogen down-regulated gene 1 protein; hexamethylene bis-acetamide inducible 1; hexamethylene bisacetamide-inducible protein; hexamethylene bis-acetamide-inducible protein 1; hexamethylene-bis-acetamide-inducible transcript 1; hexamthylene bis-acetamide inducible 1; HIS1; HMBA-inducible; MAQ1; menage a quatre 1; menage a quatre protein 1; protein HEXIM1
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Detection of human HEXIM1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HEXIM1 antibody orb1525442 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. HEXIM1 was also immunoprecipitated by rabbit anti-HEXIM1 antibodies, which recognize other epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).
Detection of human HEXIM1 by immunohistochemistry. Samples: FFPE serial sections of human lung cancer. Antibody: Affinity purified rabbit anti-HEXIM1 used at a dilution of 1: 200 (1µg/ml) or 1: 1, 000 (1µg/ml). Detection: DAB. Counterstain: hematoxylin (blue).
Detection of mouse HEXIM1 by immunohistochemistry.Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-HEXIM1 (Cat. No. orb1525442) used at a dilution of 1: 200 (1µg/ml). Detection: DAB
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_006451.1 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IP
Immunoprecipitation
Rabbit HEXIM1 Antibody (orb1525442)
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