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Rabbit PAF49 Antibody
SKU: orb1528666
Description
Images & Validation
−Item 1 of 3
| Tested Applications | ICC, IHC, IP, WB |
|---|---|
| Dilution Range | WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.; ICC-IF - 1:50 - 1:250. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells. |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 425 and 475 |
| Target | PAF49 |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 200 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−A34.5; antisense to ERCC-1 protein; ASE1; ASE-1; CAST; CD3e antigen, epsilon polypeptide associated protein; CD3e molecule, epsilon associated protein; CD3E-associated protein; CD3-epsilon-associated protein; DNA-directed RNA polymerase I subunit RPA34; PAF49; RNA polymerase I-associated factor PAF49
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Detection of human PAF49 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-PAF49 used at a dilution of 1:200 (1µg/ml).
Detection of human PAF49 by WB and IP.Samples: HeLa cells. Antibodies: Affinity purified rabbit anti-PAF49 antibody orb1528666 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate.
Detection of human PAF49 by ICC. Sample: HeLa cells. Antibody: Affinity purified rabbit anti-PAF49 used at a dilution of 1:100. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_036231.1 |
|---|
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ICC
Immunocytochemistry
IP
Immunoprecipitation
Rabbit PAF49 Antibody (orb1528666)
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