Rabbit anti-PVRL1/NECTIN1 Recombinant Monoclonal Antibody

SKU: orb1784567

Description

Images & Validation

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Item 1 of 3

Tested Applications	FC, IP, WB
Dilution range	WB: 1:1000, IP: 6 µl/mg lysate
Reactivity	Human
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Clone No.	BLR274L
Immunogen	Residues 31-331 (ECD)
Target	PVRL1/NECTIN1

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Whole IgG
Buffer/Preservatives	Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
Concentration	1000 µg/ml
Disclaimer	For research use only

Alternative Names

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PVRL1; nectin-1; OFC7; poliovirus receptor-like 1; poliovirus receptor-related 1 (herpesvirus entry mediator C); poliovirus receptor-related protein 1; PRR; SK-12; Nectin cell adhesion molecule 1; PVRR; PVRR1; CLPED1; PRR1; ectodermal dysplasia 4 (Margarita Island type); nectin 1; ED4; CD111; herpes simplex virus type 1 sensitivity; herpes virus entry mediator C; Herpesvirus entry mediator C; herpesvirus Ig-like receptor; HIgR; HV1S; HVEC; CD antigen CD111

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Rabbit anti-PVRL1/NECTIN1 Recombinant Monoclonal Antibody

Detection of human PVRL1/NECTIN1 by western blot. Samples: Whole cell lysate (50 µg) from MCF-10A, SNB-75, RT-4, MCF-7, and HaCaT cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PVRL1/NECTIN1 recombinant monoclonal antibody (orb1784567) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human PVRL1/NECTIN1 (shaded) in FaDu cells by flow cytometry. Antibody: Rabbit anti-PVRL1/NECTIN1 recombinant monoclonal antibody (orb1784567) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human PVRL1/NECTIN1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MCF-10A cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-PVRL1/NECTIN1 recombinant monoclonal antibody (orb1784567) used for IP at 6 µl/mg lysate.

Quick Database Links

Gene Symbol

PVRL1/NECTIN1

UniProt

Q15223

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

Flow Cytometry

Immunoprecipitation