Rabbit anti-PVRL1/NECTIN1 Recombinant Monoclonal Antibody

SKU: orb1806452

Description

Images & Validation

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Tested Applications	WB
Dilution range	WB: 1:1000
Reactivity	Human, Mouse
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Clone No.	BLR284L
Immunogen	Between 225 and 275
Target	PVRL1/NECTIN1
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Whole IgG
Buffer/Preservatives	Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	500 µg/ml
Disclaimer	For research use only

Alternative Names

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PVRL1; nectin-1; OFC7; poliovirus receptor-like 1; poliovirus receptor-related 1 (herpesvirus entry mediator C); poliovirus receptor-related protein 1; PRR; SK-12; Nectin cell adhesion molecule 1; PVRR; PVRR1; CLPED1; PRR1; ectodermal dysplasia 4 (Margarita Island type); nectin 1; ED4; CD111; herpes simplex virus type 1 sensitivity; herpes virus entry mediator C; Herpesvirus entry mediator C; herpesvirus Ig-like receptor; HIgR; HV1S; HVEC; CD antigen CD111

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Rabbit anti-PVRL1/NECTIN1 Recombinant Monoclonal Antibody

Detection of human PVRL1/NECTIN1 by western blot. Samples: Whole cell lysate (10 µg) from MCF-10A, SNB-75, HaCaT, MCF-7, and RT-4 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PVRL1/NECTIN1 recombinant monoclonal antibody (orb1806452) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of mouse PVRL1/NECTIN1 by western blot. Samples: Whole cell lysate (50 µg) from ND7/23, C6, RAW 264.7, J774A1, and AML12 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PVRL1/NECTIN1 recombinant monoclonal antibody (orb1806452) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Quick Database Links

Gene Symbol

PVRL1/NECTIN1

UniProt

Q15223

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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