Rabbit SET7 Antibody

SKU: orb1527863

Description

SET7 Antibody

Research Area

Neuroscience

Images & Validation

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Item 1 of 2

Tested Applications	IHC, IP, WB
Dilution range	WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Reactivity	Human, Mouse
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 316 and C-terminus
Target	SET7
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	200 µg/ml
Disclaimer	For research use only

Alternative Names

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H3-K4-HMTase SETD7; histone H3-K4 methyltransferase SETD7; histone H3-lysine 4-specific methyltransferase; histone-lysine N-methyltransferase SETD7; KMT7; lysine N-methyltransferase 7; SET domain-containing protein 7; SET7; SET7/9; SET9

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Detection of human and mouse SET7 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) , and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-SET7 antibody orb1527863 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Detection of human SET7 by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-SET7 (Cat. No. orb1527863) used at a dilution of 1: 1, 000 (0.2 µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

SET7

UniProt

Q8WTS6

RefSeq (Protein):NP_085151.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_085151.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunoprecipitation

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