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Rabbit anti-ZEB1 Antibody
SKU: orb1527548
Description
Images & Validation
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| Tested Applications | IHC, IP, WB |
|---|---|
| Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:200 - 1:1000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Between 1074 and C-terminus |
| Target | ZEB1 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Whole IgG |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 200 µg/ml |
| Disclaimer | For research use only |
Alternative Names
−PPCD3; posterior polymorphous corneal dystrophy 3; zinc finger homeodomain enhancer-binding protein; zinc finger E-box-binding homeobox 1; ZFHX1A; ZFHEP; transcription factor 8 (represses interleukin 2 expression); Transcription factor 8; TCF-8; AREB6; NIL-2-A zinc finger protein; NIL2A; negative regulator of IL2; FECD6; DELTAEF1; delta-crystallin enhancer binding factor 1; BZP; TCF8
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Detection of human and mouse ZEB1 by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, HeLa, A-549, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ZEB1 antibody orb1527548 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human ZEB1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZEB1 antibody orb1527548 used for IP at 6 µg per reaction.
Detection of mouse ZEB1 by immunohistochemistry.Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-ZEB1 (Cat. No. orb1527548) used at a dilution of 1: 200 (1µg/ml). Detection: DAB
Detection of human ZEB1 by immunohistochemistry.Sample: FFPE section of humanglioblastoma. Antibody: Affinity purified rabbit anti-ZEB1 (Cat. No. orb1527548) used at a dilution of 1: 200 (1µg/ml). Detection: DAB
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_110378.3 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IP
Immunoprecipitation
Rabbit anti-ZEB1 Antibody (orb1527548)
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