Rabbit Calretinin (CR) ELISA Kit

SKU: orb782850

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Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cr(Creatinine) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cr(Creatinine).

Research Area

Cancer Biology, Neuroscience

Images & Validation

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Tested Applications

ELISA

Application Notes

standard: 80 nmol/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rabbit CR protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit CR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit CR in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Rabbit
Sample Types	Serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay Type	Competitive
Assay Time	2h
Range	1.25-80 nmol/mL
Sensitivity	0.74 nmol/mL
Concentration	80 nmol/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CR ELISA Kit, Calretinin ELISA Kit, CALB2 ELISA Kit, CAB29 ELISA Kit, CAL2 ELISA Kit, 29 kDa calbindin ELISA Kit, calbindin 2 ELISA Kit, calbindin 2 ELISA Kit, ELISA Kit, 29kD ELISA Kit, calretinin ELISA Kit, calbindin D29K ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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