Rabbit CD86 protein

SKU: orb707312

Description

Recombinant Rabbit CD86 Protein is produced by mammalian expression system and the target gene encoding Ala23-Arg246 is expressed with a 6His tag at the C-terminus.

Research Area

Epigenetics, Immunology

Images & Validation

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Tested Applications	ELISA, MS, SDS-PAGE, WB
Application Notes	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

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Source	Human cells
Reactivity	Rabbit
Protein Sequence	AASLRIQAYF NKTADLPCQF TNSQSRSLSE LVVFWQDQER LVLYELFLGR EKPDNVDPKY IGRTSFDQES WNLQLHNVQI KDKGVYQCFV HHRGAKGLVP IYQMNSELSV LANFTQPEIT LISNITRNSA INLTCSSVQG YPEPKKMFFV LKTENATTEY DGVIEKSQDN VTGLYNISIS GSITFSDDIR NATIYCVLQT ESTETYSQHF PIVPADPVPV EKPRHHHHHH
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Endotoxins	Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.

Storage & Handling

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Storage	Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/Appearance	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Disclaimer	For research use only

Alternative Names

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CD28LG2; T-lymphocyte activation antigen CD86; Activation B7-2 antigen; B70; BU63; CTLA-4 counter-receptor B7.2; FUN-1; CD86

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Quick Database Links

UniProt

P42071

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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SDS-PAGE

Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis

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