Rabbit IgM (mu) Antibody Peroxidase Conjugated

SKU: orb346690

Description

Rabbit IgM (mu) antibody (Peroxidase)

Images & Validation

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Tested Applications	ELISA, IHC, WB
Dilution range	ELISA: 1:20,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
Reactivity	Rabbit
Application Notes	Rabbit IgM (mu) peroxidase conjugated antibody has been tested by ELISA and is assayed against 1.0 ug of Rabbit IgM in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:40,000 of the reconstitution concentration is suggested for this product.

Key Properties

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Antibody Type	Secondary Antibody
Host	Goat
Clonality	Polyclonal
Isotype	IgG
Immunogen	Anti-Rabbit IgM (mu) was produced by repeated immunization with rabbit IgM mu heavy chain in goat.
Purity	This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by cross adsorption to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgM and Rabbit Serum. No reaction was observed against Rabbit IgG.
Conjugation	HRP

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Concentration	10.0 mg/mL
Disclaimer	For research use only

Alternative Names

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Rabbit IgM (mu) Antibody, Gt-a-Rabbit IgM (mu) Peroxidase conjugated, Rabbit IgM (mu) Peroxidase Antibody in goat, Goat peroxidase conjugated Secondary Antibody, goat anti-rabbit IgM (mu chain) HRP conjugated antibody

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Rabbit IgM (mu) Antibody Peroxidase Conjugated

ELISA Results of Goat Anti-Rabbit IgM Antibody Peroxidase Conjugated. Each well was coated in duplicate with 1.0 µg of Rabbit IgM (p/n orb346313). The working dilution is 39000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate (p/n orb348651).

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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