Rabbit NLR Family, Pyrin Domain Containing Protein 3 (NLRP3) ELISA Kit

SKU: orb866965

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Description

Sandwich Rabbit NLRP3 ELISA Kit

Research Area

Signal Transduction

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit NLRP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit NLRP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit NLRP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit NLRP3 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Rabbit
Sample Types	Tissue homogenates, cell lysates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.32-20 ng/mL
Sensitivity	0.108 ng/mL
Concentration	20 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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AVP ELISA Kit, AGTAVPRL ELISA Kit, AII ELISA Kit, AII/AVP ELISA Kit, C1orf7 ELISA Kit, CIAS1 ELISA Kit, CLR1.1 ELISA Kit, FCAS ELISA Kit, FCU ELISA Kit, MWS ELISA Kit, NALP3 ELISA Kit, PYPAF1 ELISA Kit, Cryopyrin ELISA Kit, Cold Induced Autoinflammatory Syndrome 1 ELISA Kit, Caterpiller-Like Receptor 1.1 ELISA Kit

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Rabbit NLR Family, Pyrin Domain Containing Protein 3 (NLRP3) ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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