Rabenosyn-5 rabbit pAb Antibody

SKU: orb766172

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Description

Rabbit polyclonal antibody to Rabenosyn-5

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, IHC, WB
Dilution range	WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Reactivity	Human, Mouse

Key Properties

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Clonality	Polyclonal
Isotype	IgG
Immunogen	The antiserum was produced against synthesized peptide derived from human ZFYVE20. AA range:145-194
Molecular Weight	89
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Anti-ZFYVE20 antibody, anti-Rabenosyn-5 antibody, anti-110 kDa protein antibody, anti-FYVE finger-containing Rab5 effector protein rabenosyn-5 antibody, anti-Zinc finger FYVE domain-containing protein 20 antibody

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Western Blot analysis of various cells using Rabenosyn-5 Polyclonal Antibody diluted at 1:1000.

Western blot analysis of lysates from HUVEC and Jurkat cells, using ZFYVE20 Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of the lysates from COLO205 cells using ZFYVE20 antibody.

Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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