Tnfsf11 Antibody

SKU: orb345227

This product can be custom made

Description

RANK-L antibody

Research Area

Stem Cell & Developmental Biology

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, WB
Dilution Range	ELISA: 1:10,000, WB: 1:1000
Reactivity	Mouse
Application Notes	This purified antibody has been tested in western blotting and suitable for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 19-20 kDa in size corresponding to the mature mouse RANKL protein by western blotting in appropriate cell lysate or extract.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	This IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with recombinant truncated mouse RANKL protein (aa 143-316) produced in E.coli.
Target	Tnfsf11
Purity	Anti-RANK L antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for mouse RANKL protein. A BLAST analysis was used to suggest cross-reactivity with RANKL from mouse sources based on 100% homology with the immunizing sequence. Based on 97% homology, cross-reactivity with rat, 91% with Chinese hamster, 90% with Thirteen-lined ground squirrel; other sources has not been determined.
Conjugation	Unconjugated

Storage & Handling

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Storage	Store antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	Preservative: None. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.0 mg/mL
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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rabbit anti-RANK-L Antibody, Receptor activator of nuclear factor kappa B, TNF-related activation-induced cytokine, TRANCE, Tumor necrosis factor ligand superfamily member 11, TNF11, TNFRSF11, Osteoprotegerin ligand, OPG, Osteoclast differentiation factor, ODF, CD254

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Anti-mouse RANKL antibody in western blot shows detection of recombinant mouse RANKL raised in E.coli. Recombinant truncated protein (0.1 µg, 19.9 kDa) was loaded on to an SDS-PAGE gel, and after separation, transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Biorbyt's Anti-Mouse RANKL antibody diluted 1:1000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody Dylight™ 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody diluted 1:20000 in blocking buffer (p/n orb348637) for 30 min at RT.

Quick Database Links

Gene Symbol

Tnfsf11

UniProt

O35235

RefSeq (Protein):NP_035743.2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_035743.2

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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