Rat Cluster of Differentiation 3 (CD3) ELISA Kit

SKU: orb866948

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Description

Sandwich Rat CD3 ELISA Kit

Research Area

Immunology & Inflammation, Signal Transduction

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CD3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CD3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CD3 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Rat
Sample Types	Tissue homogenates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	0.32-20 ng/mL
Sensitivity	0.124 ng/mL
Concentration	20 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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CD3 ELISA Kit, CD3e antigen ELISA Kit, CD3e antigen ELISA Kit, epsilon polypeptide ELISA Kit, TiT3 complex ELISA Kit, CD3e molecule ELISA Kit, epsilon ELISA Kit, CD3-TCR complex ELISA Kit, CD3-epsilon ELISA Kit, FLJ18683 ELISA Kit, T3E ELISA Kit, T-cell antigen receptor complex ELISA Kit, epsilon subunit of T3 ELISA Kit, T-cell surface antigen T3 ELISA Kit, Leu-4 epsilon chain ELISA Kit, T-cell surface glycoprotein CD3 epsilon chain ELISA Kit, TCRE ELISA Kit

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Rat Cluster of Differentiation 3 (CD3) ELISA Kit

Quick Database Links

UniProt

P04235

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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