Rat Complement C5 Protein, His Tag

SKU: orb570266

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Description

Rat Complement C5, His Tag is expressed from human 293 cells (HEK293). It contains AA Gln 24 - Ala 1688 (Accession # XP_001079130.2).

Research Area

Immunology & Inflammation

Images & Validation

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Application Notes

This protein carries a polyhistidine tag at the C-terminus. The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 74.1 kDa (β chain) and 114.8 kDa (α chain). The protein migrates as 70 kDa and 110 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

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Expression System	HEK293
Target	Complement C5
Reactivity	Rat
Tag	C-10×His
Molecular Weight	74.1 kDa (β chain) & 114.8 kDa (α chain)
Expression Region	Gln 24 - Arg 683 & Asp 684 - Ala 1688
Purity	95%
Endotoxins	1.0 EU per μg
Conjugation	Unconjugated

Storage & Handling

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Storage	-20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
Form/Appearance	Powder
Buffer/Preservatives	PBS, pH 7.4
Disclaimer	For research use only

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Immobilized Rat Complement C5, His Tag (Cat. No. orb570266) at 2 μg/mL (100 μL/well) can bind Anti-C5a (Human IgG1) with a linear range of 0.4-12.5 ng/mL (QC tested).

Quick Database Links

Gene Symbol

Complement C5

UniProt

XP_001079130.2

UniProt Details

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No UniProt data available

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