Rat Factor Related Apoptosis Ligand (FASL) ELISA Kit

SKU: orb779094

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Factor Related Apoptosis Ligand(FASL).

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Tested Applications

ELISA

Application Notes

standard: 500 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat FASL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat FASL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat FASL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat FASL in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Rat
Sample Types	Serum, plasma, tissue homogenates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	7.82-500 pg/mL
Sensitivity	3.4 pg/mL
Concentration	500 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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CD178 ELISA Kit, CD95L ELISA Kit, CD95-L ELISA Kit, FASLG ELISA Kit, FASLG ELISA Kit, APT1LG1 ELISA Kit, APT1-LG1 ELISA Kit, TNFSF6 ELISA Kit, Fas Antigen Ligand ELISA Kit, TNF Superfamily Member 6 ELISA Kit, Tumor Necrosis Factor(ligand)Superfamily,Member 6 ELISA Kit

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Rat Factor Related Apoptosis Ligand (FASL) ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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