Rat Heparan Sulfate Proteoglycan (HSPG) ELISA Kit

SKU: orb781558

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heparan Sulfate Proteoglycan(HSPG).

Research Area

Cancer Biology, Infectious Disease & Virology, Metabolism Research, Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HSPG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HSPG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HSPG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HSPG in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Rat
Sample Types	serum, plasma, tissue homogenates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	31.25-2000 pg/mL
Sensitivity	12.2 pg/mL
Concentration	2000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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SDC2 ELISA Kit, HSPG1 ELISA Kit, SYND2 ELISA Kit, Fibroglycan ELISA Kit, Syndecan 2 ELISA Kit, Syndecan Proteoglycan 2 ELISA Kit, Heparan Sulfate Proteoglycan 1,Cell Surface-Associated ELISA Kit, Heparan Sulphate Proteoglycan ELISA Kit

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Rat Heparan Sulfate Proteoglycan (HSPG) ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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