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Rat IgG (H&L) Antibody
SKU: orb347848
Description
Images & Validation
−Item 1 of 1
| Tested Applications | DOT, ELISA, IHC, WB |
|---|---|
| Dilution range | ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000 |
| Reactivity | Rat |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Rat IgG whole molecule |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Rat IgG and Rat Serum. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Concentration | 2.25 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-Rat IgG (H&L) Antibody, Rabbit-a-Rat IgG (H&L), Rat IgG Antibody in rabbit, rabbit anti Rat IgG Secondary Antibody
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Western Blot of Anti-Rat IgG (H&L) (RABBIT) Antibody (p/n orb347848). Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule (p/n orb2652744). Lane 2: Rat IgG F(c) Fragment (p/n orb346338). Lane 3: Rat IgG Fab Fragment (p/n orb346340). Lane 4: Rat IgM Whole Molecule (p/n orb346341). All samples were reduced. Load: 50 ng per lane. Block: orb348637 for 30 min at RT. Primary Antibody: Anti-Rat IgG (H&L) (RABBIT) Antibody (p/n orb347848) 1:1000 for 60 min at RT. Secondary Antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody (p/n orb347654) 1:40000 in orb348637 for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
DOT
Dot Blot
Rat IgG (H&L) Antibody (orb347848)
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